G-FORSCHT 2015

SPECTROSCOPIC METHODS IN WINE SCIENCES Spectroscopic methods provide a fast, precise and environ- mental friendly alternative to the classical analytical techniques. Methods such as FTIR (Fourier Transform Infra- red Spectroscopy) and NMR (Nuclear Magnetic Resonance) spectroscopy are non-destructive holding a very high preci- sion. Beside the simple and rapid routine analysis, the main positive argument is the almost complete absence of re- agents and chemicals. No sample preparation, dilution or other additional steps are necessary, minimizing the source of errors. At present, FTIR spectroscopy has the possibility to analyse 14 ingredients including free and total SO2 within 30 seconds. Another advantage of spectroscopy is the good comparability of analytical results across national bor- ders. The results are comparable worldwide, and can be checked anytime and anywhere based on their raw data. Moreover, a non-targeted analysis of origin, variety, proces- sing, artefacts and adulterations is possible. Each spectrum of any individual sample represents a unique fingerprint. AUTHOR: Dr. Claus-Dieter Patz Institut Weinanalytik und Getränkeforschung claus-.patz@hs-gm.de ABSTRACTS CURRENT RESEARCH PROJECTS RUBUS STUNT: DETECTION AND MANAGEMENT OF A PHYTOPLASMA DISEASE IN RUBUS SPECIES The phytoplasma disease Rubus stunt is a major challenge in the production of raspberries and blackberries. Phyto- plasmas are cell wall-less bacteria that inhabit the phloem as obligatory parasites and are transferred by phloem feeding insects or by vegetative propagation of infected plants. Symptoms range from stunted growth, flower prolife- ration, enlarged sepals, small leaves, and short internodes to fruit malformations. Little is known about the distribution of phytoplasmas in different Rubus cultivars as well as on the spectrum of putative insect vectors. Moreover, Rubus stunt has a very long incubation period of up to one year, making propagation of infected plant material quite likely. Currently, nested PCR is used as the prevalent phytoplasma detection method, which is time consuming and prone to contamination. In this project, a rapid new molecular detec- tion method based on a multiplex TaqMan qPCR assay was developed, which is now in use for identifying putative insect vectors, for analyzing the susceptibility of different cultivars and for evaluating heat treatment for phytoplasma elimination during vegetative propagation. AUTHORS: Prof. Dr. Annette Reineke Institut Phytomedizin annette.reineke@hs-gm.de Holger Linck M.Sc. Institut Phytomedizin Dr. Erika Krüger Institut Obstbau

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